Salmonella Invasion cDNA and Antigen

Salmonella enterica is a bacterial pathogen that can cause a wide range of diseases in humans and animals, including gastroenteritis, typhoid fever, and septicemia. One of the key virulence factors of Salmonella enterica is a transcriptional regulator called InvE.

InvE is essential for the expression of the type III secretion system (T3SS), which is a critical virulence factor that allows Salmonella to inject bacterial effectors directly into host cells. These effectors manipulate the host cell machinery and allow the bacteria to survive and replicate within the host.

Because InvE is necessary for the expression of T3SS, it is an attractive target for diagnosis and treatment of Salmonella infections. Several studies have explored the use of InvE as a diagnostic target, including PCR-based assays and serological tests. For example, one study developed a PCR-based assay that specifically amplified the invE gene and demonstrated its sensitivity and specificity for detecting Salmonella enterica in food samples.

Overall, InvE is an important virulence factor for Salmonella enterica and has potential as a target for diagnosis and treatment of Salmonella infections.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

The cDNA (complementary DNA) and recombinant antigen of S. enterica can be used in various applications for diagnosis, research, and vaccine development:

Diagnostic Tests: cDNA of S. enterica can be used in molecular diagnostic tests to detect the presence of the bacterium in a patient’s sample or in food samples. This can be done by amplifying a specific genetic target using polymerase chain reaction (PCR) and detecting the amplified product using fluorescence or other methods.

Research: cDNA of S. enterica can be used in research studies to investigate the genetic characteristics and pathogenesis of the bacterium. Recombinant antigens can also be used to study the immune response to S. enterica infections, to identify potential vaccine candidates, and to develop new diagnostic tests.

Vaccine Development: Recombinant antigens of S. enterica can be used to develop vaccines against the bacterium. These vaccines can stimulate the production of specific antibodies that recognize and neutralize S. enterica.

In conclusion, the cDNA and recombinant antigen of S. enterica have important applications in the field of diagnostics, research, and vaccine development, and can help in the development of new and more effective ways to prevent and treat foodborne illness caused by this bacterium.