Riemerella anatipestifer cDNA and Antigen

Riemerella anatipestifer is a bacterium that can cause a wide range of infections in waterfowl and other avian species. This pathogen can lead to significant economic losses in the poultry industry and can also pose a threat to wild bird populations.

One important component of Riemerella anatipestifer is the 60 kDa chaperonin protein, which plays a key role in protein folding and stability. This protein is essential for the survival of the bacterium, as it helps to ensure that other proteins are properly folded and functional.

In addition to the chaperonin protein, Riemerella anatipestifer also possesses two other important proteins: OmpA and P25. OmpA is a surface protein that is involved in the adhesion of the bacterium to host cells, while P25 is a virulence factor that plays a role in the pathogenesis of the infection.

Understanding these important proteins is crucial for developing new treatments and preventative measures for Riemerella anatipestifer infections. By targeting these proteins, researchers may be able to develop new therapies to disrupt the ability of the bacterium to cause disease and spread within avian populations.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

Riemerella anatipestifer cDNA and recombinant antigens can be used in the diagnosis of avian diseases. These antigens can be used to detect the presence of R. anatipestifer in a sample. The cDNA can also be used to produce recombinant proteins, which can be used to create diagnostic kits or vaccines that can be used to prevent or treat infections caused by R. anatipestifer. Additionally, the cDNA can be used to generate transgenic chickens that are resistant to avian diseases caused by R. anatipestifer.