Microsporum canis cDNA and Antigen

Microsporum canis is a fungus belonging to the dermatophyte group, which can cause skin infections, known as dermatophytosis or ringworm, in humans and animals. Recent studies have identified several key antigens associated with Microsporum canis, including Epstein-Barr nuclear antigen 1 (EBNA1), antigen 2 (Ag2), expression library immunization antigen 1 (ELIA1), Microsporum canis antigen (MCA), and serologically defined colon cancer antigen 1 (SDCCAG1). These antigens have the potential to improve the accuracy and speed of Microsporum canis diagnosis.

Epstein-Barr nuclear antigen 1 (EBNA1): EBNA1 is a viral antigen produced by the Epstein-Barr virus (EBV), which has been found to cross-react with Microsporum canis. Cross-reactivity means that antibodies generated against EBNA1 can also recognize similar epitopes on Microsporum canis antigens. This cross-reactivity has been utilized in diagnostic tests to detect Microsporum canis infections in humans and animals, which can help improve the accuracy of diagnosis.

Antigen 2 (Ag2): Ag2 is a protein antigen produced by Microsporum canis, and it has been found to be immunogenic, meaning it can trigger an immune response in the host. Ag2 has been used in diagnostic tests to detect Microsporum canis infections, and it has shown promising results in improving the accuracy of diagnosis.

Expression library immunization antigen 1 (ELIA1): ELIA1 is a protein antigen identified through expression library immunization, a technique used to identify antigens that can stimulate an immune response. ELIA1 has been found to be specific to Microsporum canis, and it has been used in diagnostic tests to detect Microsporum canis infections, potentially improving the accuracy of diagnosis.

Microsporum canis antigen (MCA): MCA is a protein antigen produced by Microsporum canis, and it has been found to be immunogenic. MCA has been used in diagnostic tests to detect Microsporum canis infections, and it has shown potential in improving the accuracy of diagnosis.

Serologically defined colon cancer antigen 1 (SDCCAG1): SDCCAG1 is a protein antigen originally identified in colon cancer, but it has also been found to cross-react with Microsporum canis. Cross-reactivity with SDCCAG1 has been utilized in diagnostic tests to detect Microsporum canis infections in humans and animals, potentially improving the accuracy of diagnosis.

The use of these antigens in diagnostic tests has the potential to improve the accuracy and speed of Microsporum canis diagnosis, allowing for earlier and more effective treatment. However, further research and validation studies are needed to determine their clinical utility and optimize their diagnostic performance.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

The application of cDNA (complementary DNA) and recombinant antigens derived from M. canis has been of significant interest for the development of diagnostic and vaccine tools for the control of ringworm.

Diagnostics: cDNA from M. canis can be used to develop molecular diagnostic tools such as PCR (Polymerase Chain Reaction) assays for the rapid and specific detection of the fungus in infected individuals. This is especially useful in cases where traditional culture methods are not feasible or are slow.