Listeria monocytogenes cDNA and Antigen

Listeria monocytogenes is a gram-positive bacterium that can cause severe infections in humans, particularly in individuals with weakened immune systems. The detection and identification of this pathogen can be challenging, but recent studies have identified several key antigens that may serve as potential targets for pathogen detection.

60 kDa Chaperonin
The 60 kDa chaperonin is a heat shock protein that is essential for the proper folding of proteins in Listeria monocytogenes. This protein has been shown to be highly immunogenic, and antibodies against it have been detected in infected individuals. Therefore, the 60 kDa chaperonin has been identified as a potential biomarker for the detection of Listeria monocytogenes.

67 kDa Myosin-Cross-Reactive Antigen
The 67 kDa myosin-cross-reactive antigen (McrA) is a surface protein that is conserved across Listeria species. This antigen has been shown to elicit an immune response in infected individuals, making it a potential target for the development of diagnostic assays.

Antigens A, B, C, D
Antigens A, B, C, and D are a group of proteins that are expressed on the surface of Listeria monocytogenes. These antigens are highly immunogenic and have been shown to be conserved across different strains of the bacterium. Therefore, they have the potential to serve as reliable targets for the detection of Listeria monocytogenes.

Immunoreactive Antigen PG97
Immunoreactive antigen PG97 is a surface protein of Listeria monocytogenes that has been shown to elicit an immune response in infected hosts. This protein is highly conserved across different strains of the bacterium, making it a reliable target for the development of diagnostic assays.

Outer Surface Protein
The outer surface protein is a protein that is located on the outer surface of Listeria monocytogenes. This protein has been shown to be highly immunogenic, and antibodies against it have been detected in infected individuals. Therefore, the outer surface protein has been identified as a potential biomarker for the detection of Listeria monocytogenes.

The identification of these key antigens of Listeria monocytogenes has the potential to improve the accuracy and speed of pathogen detection. The 60 kDa chaperonin, 67 kDa myosin-cross-reactive antigen, antigens A, B, C, and D, immunoreactive antigen PG97, and outer surface protein are all promising candidates for the development of diagnostic assays that can detect the presence of Listeria monocytogenes in infected hosts. The validation and implementation of these biomarkers could lead to earlier diagnosis and treatment of Listeria monocytogenes infections, ultimately improving patient outcomes.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

Listeria monocytogenes cDNA and recombinant antigens can be used in a variety of applications, including vaccine development, diagnostic testing, and gene expression analysis. Vaccine development is the most common application of Listeria monocytogenes cDNA and recombinant antigens. These antigens can be used to produce vaccines that provide immunity against specific strains of Listeria monocytogenes. Additionally, these antigens can be used in the development of diagnostic tests for the detection of Listeria monocytogenes in food and environmental samples. Finally, these antigens can be used to study the expression of Listeria monocytogenes genes and proteins by making use of gene expression profiling techniques.