Haemophilus gallinarum cDNA and Antigen

Haemophilus gallinarum is a gram-negative bacterium that is commonly found in the upper respiratory tract of birds and mammals, including humans. Although it is typically considered a commensal organism, it has been associated with opportunistic infections in humans, particularly in immunocompromised individuals.

Hemagglutinin antigen is an important virulence factor of H. gallinarum that mediates the attachment of the bacterium to host cells. This protein is a type of adhesin that binds to specific receptors on the surface of host cells, allowing the bacterium to establish a foothold and colonize the host tissue.

Lipoprotein P4 and lipoprotein P6 are two other proteins that have been identified as significant factors in the pathogenicity of H. gallinarum. These proteins are involved in the host immune response, and act as immunomodulatory factors that can influence the inflammatory response. Lipoprotein P4 has been shown to induce the production of pro-inflammatory cytokines, while lipoprotein P6 can activate complement and enhance phagocytosis.

The potential use of these proteins as diagnostic and therapeutic targets is currently being investigated. For example, they could be used to develop more sensitive and specific diagnostic tests for H. gallinarum infections, or to design new treatments that target these virulence factors.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

These applications are:

Identification and characterization of virulence factors: The cDNA of H. gallinarum can be used to study the expression of genes involved in virulence and antibiotic resistance. This information can aid in the development of new treatments for avian infections caused by this bacterium.

Diagnostic tests: Recombinant antigens of H. gallinarum can be used as target antigens in diagnostic tests such as ELISA (Enzyme-linked Immunosorbent Assay) for the detection of avian infections caused by this bacterium.

Vaccine development: The recombinant antigens of H. gallinarum can be used as potential vaccine targets to protect against avian infections caused by this bacterium.

Antibiotic resistance studies: The cDNA of H. gallinarum can be used to study the expression of genes involved in antibiotic resistance, which can help in the development of new antibiotics to combat antibiotic-resistant strains of this bacterium.