Granulicatella adiacens cDNA and Antigen

Granulicatella adiacens is a Gram-positive bacterium that is part of the normal oral microbiota in humans. It is also known to cause various infections, such as endocarditis, bacteremia, and brain abscess. One of the surface antigens of Granulicatella adiacens is the cell surface antigen I-II (GAA I-II). This protein has been the subject of several studies exploring its significance as a membrane protein and surface antigen.

GAA I-II is a surface-exposed protein that belongs to the antigen I/II family of proteins. This family of proteins is characterized by their ability to bind to various surfaces, including host tissues and bacteria. GAA I-II has been shown to have adhesive properties and can bind to a variety of substrates, including collagen, fibronectin, and laminin. These properties suggest that GAA I-II may play a role in the pathogenesis of Granulicatella adiacens infections by facilitating bacterial attachment and colonization.

Studies have also investigated the potential of GAA I-II as a vaccine candidate. Vaccination against surface antigens is a common approach to prevent bacterial infections. Some studies have shown that antibodies against GAA I-II can prevent bacterial adhesion and colonization, indicating that this protein may be a potential vaccine candidate for Granulicatella adiacens infections.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

The application of Granulicatella adiacens cDNA and recombinant antigen in the diagnosis of endocarditis caused by Granulicatella adiacens requires the use of a specific set of tools and techniques. First, a comprehensive clinical history and physical examination must be performed to assess for any signs or symptoms of endocarditis. Following this, a blood sample must be taken and cultured to identify the presence of Granulicatella adiacens. If the organism is identified, a PCR assay can then be performed to specifically detect the presence of the organism’s cDNA. Additionally, a recombinant antigen assay can be used to detect the presence of Granulicatella adiacens-specific antigens in the sample. Both the cDNA and antigen assays can be used in conjunction to provide a more accurate diagnosis of endocarditis caused by Granulicatella adiacens.