Enterococcus faecium cDNA and Antigen

Enterococcus faecium is a Gram-positive, opportunistic pathogen found in the gastrointestinal tract of humans and other animals. It is a commensal organism that can cause a range of infections, most commonly urinary tract infections and bacteremia. Other infections associated with E. faecium include endocarditis, meningitis, and intraabdominal and wound infections. E. faecium is known to be resistant to many antibiotics, including penicillin, aminoglycosides, and vancomycin, making it a difficult organism to treat. Additionally, it can form biofilms which further complicate treatment.Enterococcus faecium possesses several important proteins, including:

Secreted antigen SagBb: A protein that is secreted by the bacterium and is believed to play a role in virulence.
Streptococcal 67 kDa myosin-cross-reactive antigen: A protein that has cross-reactivity with human myosin and is thought to be involved in the autoimmune response seen in rheumatic fever.
Acetyltransferase SatG: A protein that confers resistance to the antibiotic chloramphenicol by acetylating it.
D-alanine:D-alanine ligase: A protein that is involved in the synthesis of peptidoglycan, a key component of bacterial cell walls.
Esp: A surface protein that is involved in the adhesion of the bacterium to host cells.
Glycosidase GlyA: A protein that cleaves glycosidic bonds and is involved in the degradation of complex carbohydrates.
Hyaluronidase: A protein that degrades hyaluronic acid, a component of the extracellular matrix of host tissues.
SatG protein (VatE): A protein that confers resistance to the antibiotic streptogramin A by acetylating it.
VanA protein: A protein that is involved in vancomycin resistance, a critical issue in the treatment of enterococcal infections.
Overall, these proteins play important roles in the pathogenicity and antibiotic resistance of Enterococcus faecium.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

Enterococcus faecium cDNA and recombinant antigen can be used in a variety of applications.

1. Vaccine Development: Enterococcus faecium cDNA and recombinant antigen can be used in the development of vaccines. The cDNA can be used to produce proteins or antigens that can be used as antigens in vaccine development. The recombinant antigens can be used to stimulate an immune response in the body, which can then be used to protect against infection.

2. Diagnostics: The cDNA and recombinant antigen can be used to develop diagnostic tests to detect Enterococcus faecium. The cDNA can be used to develop PCR-based tests that can detect the presence of DNA from the bacteria. The recombinant antigens can be used to develop antibody-based tests that can detect antibodies against the bacteria.

3. Therapeutics: The cDNA and recombinant antigen can be used in the development of therapeutic agents. The cDNA can be used to produce proteins that can be used to target the bacteria and inhibit its growth. The recombinant antigens can be used to develop antibodies that can be used to target the bacteria and neutralize it.