Campylobacter fetus cDNA and Antigen

Campylobacter fetus is a species of bacteria that can cause serious illnesses in humans and animals. The bacterium is most often found in the intestines of animals and may be present in contaminated food or water. The antigen of Campylobacter fetus is a protein which is used in laboratory tests to identify the presence of the bacterium. These tests are used to detect the presence of Campylobacter in food or water samples, as well as in patients suspected of having a Campylobacter infection.The 60 kDa chaperonin Cpn60 and invasion antigen B are important proteins in Campylobacter fetus.

Cpn60 is a chaperone protein that plays a crucial role in protein folding and cellular stress response. It is also involved in the assembly of some macromolecular complexes. In Campylobacter fetus, Cpn60 has been shown to be a potent antigen, inducing a strong immune response in infected individuals.

Invasion antigen B is a protein that is involved in the virulence of Campylobacter fetus. It is thought to be involved in the initial attachment and invasion of host cells by the bacteria. The protein has been shown to be immunogenic and can stimulate an immune response in infected individuals.

Understanding the properties and functions of these proteins is important for developing treatments and vaccines against Campylobacter fetus infections.

Campylobacter fetus cDNA and recombinant antigen can be used in a variety of applications. One example is a diagnostic test for C. fetus infections. For example, a recombinant C. fetus antigen could be used in an ELISA test. In this test, the antigen is immobilized on a solid phase and then specific antibodies that recognize the antigen can be detected in a sample. This can be used to quickly and accurately diagnose an infection.

Additionally, the cDNA of C. fetus can be used to produce the antigen in large quantities. This can be used for vaccine development, as it can be used to mass-produce the antigen for use in a vaccine.

Lastly, the cDNA can be used to study the genetic makeup of the organism. The sequence of the cDNA can be used to identify the genes present in the organism, and it can also be used to identify the regulatory elements that control gene expression. This can be used to develop new strategies for fighting infections caused by C. fetus, as well as to study the biology of the organism.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.