Aliivibrio salmonicida cDNA and Antigen

Alivivibriosalmonicida is a Gram-negative, rod-shaped, obligate aerobe bacterium. It is motile, with a single polar flagellum, and is typically found in aquatic environments, such as brackish water, marine environments and ponds. It is a member of the Vibrionaceae family and is closely related to other species within the Alivivibrio genus, such as A. fischerii and A. fibrillosus. It is a common pathogen of fish and is responsible for a number of diseases in farmed salmon and other fish species, such as furunculosis and vibriosis. It is also known to cause mortality in wild salmon populations.

As aquatic environments continue to face increasing stress from climate change, pollution, and other human activities, the health of aquatic organisms has become more important than ever before. One key aspect of maintaining healthy aquatic organisms is understanding the role of proteins like Aliivibrio salmonicidac Extracellular Solute-Binding Protein (ESBP) and Surface Antigen Protein A (SapA) in the health and survival of aquatic species.

Aliivibrio salmonicidac ESBP

Aliivibrio salmonicidac ESBP is an essential protein found in a range of aquatic organisms, including fish, shrimp, and other marine species. ESBP plays a crucial role in maintaining the balance of solutes in the extracellular environment of these organisms, which is critical for maintaining their overall health and survival.

One of the key functions of ESBP is to act as a carrier protein, binding to specific solutes and transporting them across the cellular membrane. This process is essential for maintaining the proper concentration of solutes in the extracellular environment, which helps to prevent dehydration and other cellular stresses.

Aliivibrio salmonicidacSapA

Another important protein in aquatic health is Aliivibrio salmonicidacSapA. This surface antigen protein plays a crucial role in the immune response of aquatic organisms, helping to protect them from a range of bacterial and viral pathogens.

SapA is an essential component of the outer membrane of Aliivibrio salmonicidac and other aquatic organisms. When pathogens enter the extracellular environment, SapA recognizes and binds to these pathogens, triggering a series of immune responses that help to neutralize the threat and protect the organism from harm.

Importance of Proteins for Aquatic Health

The importance of proteins like ESBP and SapA for maintaining the health and survival of aquatic organisms cannot be overstated. These proteins play critical roles in maintaining proper solute balance, preventing dehydration and other cellular stresses, and protecting against a range of bacterial and viral pathogens.

The use of recombinant proteins/cDNA in academic research and diagnostic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.