Epizootic haematopoietic necrosis virus cDNA and recombinant antigen

Epizootic haematopoietic necrosis virus (EHNV) is a species of the Rhabdoviridae family of viruses, which causes a highly infectious and fatal disease in fish species. EHNV is known to affect a wide range of fish species including salmon, trout, and eel, and is widely distributed across Australia, New Zealand, and the Pacific region. The virus is primarily spread through the movement of infected fish, but can also be spread through contaminated water or equipment.

Epizootic Haematopoietic Necrosis Virus (EHNV) is a double-stranded DNA virus that infects fish and causes severe anemia and necrosis of the hematopoietic tissues. EHNV encodes for several proteins, including the P31 and P40 proteins, as well as the major capsid protein.

The Epizootic haematopoietic necrosis virus (EHNV) antigen includes a specific protein or molecule present on the surface of the virus that can be recognized by the host’s immune system. Antigens play a crucial role in the host-virus interaction, as they are used by the immune system to identify and target invading pathogens. In the case of EHNV, the antigenic properties of the virus can be used to develop diagnostic tests, such as antibody-based assays, that can detect the presence of the virus in infected fish populations. Additionally, the study of EHNV antigens can provide important information about the evolution and diversity of the virus, as well as the mechanisms underlying its pathogenesis. Understanding the antigenic properties of EHNV is important for the development of effective control measures and vaccines, as well as for the management and prevention of EHNV infections in fish populations. The virus genome encodes several proteins, including:

P31: This is a non-structural protein that is involved in viral replication and assembly.

Major capsid protein: This is a structural protein that forms the outer shell of the virus and is involved in protecting the viral genome and facilitating viral entry into host cells.

P40: This is another structural protein that is involved in the assembly of the virus particles.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.