Bovine enteric calicivirus cDNA and Antigen

Bovine enteric calicivirus (BECV) is a pathogenic virus that can cause a viral disease in cattle. BECV is a member of the Caliciviridae family and can cause diarrhea and dehydration in cattle, leading to decreased weight gain, decreased milk production, and decreased fertility. BECV is highly contagious and can spread rapidly through cattle populations, making it important to take preventive measures such as vaccination and biosecurity measures to control BECV infections in cattle populations.

Bovine enteric calicivirus (BECV) antigen refers to a substance that is recognized by the immune system as foreign and triggers the production of antibodies. This antigen is found in the BECV virus and can be used in laboratory tests to detect BECV infections in cattle. Detection of BECV antigen in blood or serum samples can help diagnose BECV infections and monitor the effectiveness of vaccines against the virus.

Bovine enteric calicivirus (BECV) is a non-enveloped, positive-sense, single-stranded RNA virus that infects cattle. BECV has several structural and non-structural proteins, including:

Capsid protein: This protein is a structural protein that encapsidates the viral RNA genome. It is involved in virus assembly and is a target for the immune response.

VP2 protein: This protein is a structural protein that is part of the viral capsid. It plays a key role in virus assembly and is a target for the immune response.

VP1 protein: This protein is a structural protein that is part of the viral capsid. It is involved in virus assembly and is a target for the immune response.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

The application of BECV cDNA and recombinant antigens has been explored as a potential tool for the diagnosis, treatment and prevention of BECV infection. Recombinant antigens can be used to detect BECV infection in cattle, and cDNA has been successfully used to create a vaccine for the virus. Additionally, cDNA can be used to identify new BECV variants, which can help researchers understand the virus and develop new treatments. Finally, cDNA can be used to create a recombinant BECV antigen-based diagnostic assay, allowing for more accurate diagnosis and treatment of BECV infections in cattle.