Bovine Delphinium virus cDNA and Antigen

Bovine Delphinium virus (BDV) is a pathogenic virus that can cause a viral disease in cattle. BDV is a member of the Nodaviridae family and causes symptoms such as fever, weight loss, and decreased milk production. BDV infections can have a significant economic impact on the livestock industry, leading to decreased weight gain, decreased milk production, and decreased fertility. It is important to take preventive measures such as vaccination and biosecurity measures to control BDV infections in cattle populations.

Bovine Delphinium virus (BDV) antigen refers to a substance that is recognized by the immune system as foreign and triggers the production of antibodies. This antigen is found in the BDV virus and can be used in laboratory tests to detect BDV infections in cattle. Detection of BDV antigen in blood or serum samples can help diagnose BDV infections and monitor the effectiveness of vaccines against the virus.

The genome of Bovine Delphinium virus (BDV) is the complete genetic material of the virus. It is composed of a single-stranded RNA (ribonucleic acid) molecule and is approximately 4 kilobases in length. The BDV genome encodes for several proteins that are involved in virus replication, assembly, and pathogenesis. Understanding the BDV genome can help in the development of diagnostic tools and vaccines for the control of BDV infections in cattle. The genome can also be used to study the genetic diversity and evolution of BDV in different cattle populations.

Glycoprotein: This protein is a surface protein that is involved in viral attachment and entry into host cells.

P40: This protein is a non-structural protein that is involved in viral replication and transcription.

N protein: This protein is a structural protein that is involved in encapsidation of the viral RNA genome.

P24: This protein is a non-structural protein that is involved in viral replication and transcription.

P16: This protein is a non-structural protein that is involved in viral replication and transcription.

P10: This protein is a non-structural protein that is involved in viral replication and transcription.

The recombinant antigen can be used in an ELISA or competitive ELISA to detect antibodies to BDV in cattle serum samples. This allows for the rapid diagnosis of BDV infection and helps to identify infected animals in herds. The recombinant antigen can also be used in a vaccine to protect cattle from BDV infection. The vaccine is made up of the recombinant antigen and an adjuvant and has been shown to provide protection against BDV infection in cattle.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.