Avian Infectious Bronchitis Virus cDNA and Antigen

Avian Infectious Bronchitis Virus (IBV) is a member of the Coronaviridae family, and its genome is composed of a single-stranded RNA. The virus primarily targets the respiratory and urogenital tracts of chickens, causing respiratory distress, reduced egg production, and poor egg quality.

Spike proteins S1 and S2 are the major surface proteins of IBV and are involved in the binding and entry of the virus into host cells. S1 recognizes and binds to host cell receptors, while S2 is responsible for fusion of the viral and host cell membranes.

VP1 is a viral RNA-dependent RNA polymerase that is involved in the replication and transcription of the viral genome. The Structural polyprotein is involved in virus assembly and includes several structural proteins, such as the nucleocapsid protein and membrane protein.

Viral protein 5 is a non-structural protein that is involved in viral replication and pathogenesis. It plays a role in inhibiting the host immune response, allowing the virus to establish infection and evade clearance by the host.

Understanding the role of these key IBV proteins is essential for the development of effective control measures against this economically important disease. Ongoing research efforts aim to identify new targets for antiviral drugs and to develop improved vaccines to protect chickens from IBV infection.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

Avian infectious bronchitis virus cDNA and recombinant antigens can be used for the development of diagnostic assays and vaccines. For example, the cDNA can be used to generate recombinant antigens which can then be used to develop diagnostic assays such as ELISA or Western blotting which can be used to detect the presence of the virus in an infected bird. The recombinant antigens can also be used to develop a vaccine which can be used to protect birds from infection. In addition, the cDNA can be used to create transgenic birds which are resistant to the virus, which can help reduce the spread of the virus in poultry flocks.