BcMag™ One-Step Mosquitoes DNA Purification Kit

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BcMag™ One-Step Mosquitoes DNA Purification Kit: A Rapid and Efficient Solution for Insect Genomic DNA Extraction

Introduction

Mosquito-borne diseases refer to infectious illnesses transmitted through the bite of infected mosquitoes. Notable examples of diseases spread to humans via mosquitoes encompass Zika virus, West Nile virus, Chikungunya virus, dengue, and malaria. Understanding the transmission dynamics and clinical aspects of these diseases is of paramount importance in devising effective prevention and control strategies, necessitating continuous research and collaborative efforts in the field of public health.

Efficient DNA extraction from mosquitoes is crucial for a myriad of research applications, including plant genotyping and molecular studies. However, conventional bind-wash-elute procedures have proven to be time-consuming and demanding, presenting challenges related to DNA loss and degradation. The BcMag™ One-Step Mosquitoes DNA Purification Kit represents a groundbreaking advancement in this field, streamlining the genomic DNA extraction process and alleviating the limitations of traditional techniques.

Methodology

The BcMag™ Kit utilizes proprietary magnetic beads and buffers specifically designed for effective cell lysis and simultaneous PCR inhibitors removal in an aqueous buffer, leaving the DNA untouched. This innovative approach minimizes the risk of DNA degradation and circumvents the need for harsh lysis conditions, such as alkaline lysis and toxic chemicals, preserving DNA integrity. Furthermore, the avoidance of organic solvents during the lysis process eliminates the time-consuming cleanup step, simplifying the overall DNA extraction procedure.

Collect and add the sample (1-2 Mosquito,0.5 mg-1mg) to PCR tube.

Mix the samples with the magnetic beads and proteinase K and heat to lyse the cells.

Vortex beads capture PCR inhibitors.

Remove the beads with a magnet.

Aspirate the supernatant containing the pure ready-to-use DNA.

Performance

The purified DNA is ready for downstream applications, such as PCR, qPCR, single-nucleotide polymorphism (SNP), short tandem repeat (STR) genotyping, genotyping, next-generation sequencing (NGS, veterinary genotyping, etc.

Features and Advantages

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Rapid and efficient extraction protocol: without prior DNA isolation for subsequent use in direct workflows, No liquid transfer, and One-tube.

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Ultrafast: Process 96 samples in less than an hour.

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Highest nucleic acids recovery rates: Minimal loss of DNA during extraction

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Effectively cell lysate cleanup and removes inhibitors: polyphenolic compounds, humic/fulvic acids, acidic polysaccharides, tannins, melanin, heparin, detergents, denim dyes, divalent cations such as Ca²⁺, Mg²⁺, etc.

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Cost-effective: Eliminates columns, filters, laborious repeat pipetting, and organic reagents.

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High-throughput: Compatible with many different automated liquid handling systems.

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One-Step DNA Purification

Bioclone