Proliferative Kidney Organism Antigen PKX101 is a protein antigen found in the parasitic organism, Heteropolaria sp., that infects the kidneys of fish. This antigen is of significant interest to researchers because it triggers an immune response in infected fish, leading to the formation of granulomas in the kidneys. These granulomas are characterized by the proliferation of fibroblasts and macrophages, which can cause severe damage to the kidney tissue.

Despite the damage caused by PKX101, it is a useful tool in the study of immune responses in fish. Researchers are interested in understanding the mechanisms of the immune response to this antigen and how it can be used to protect fish against similar infections. The antigen is also being investigated as a potential therapeutic target for treating fish diseases.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.