Babesia gibsoni is a tick-borne hemoprotozoan parasite that infects dogs, causing canine babesiosis. The disease is characterized by fever, anemia, jaundice, and hemoglobinuria, and can be fatal if left untreated. Babesia gibsoni is transmitted by the brown dog tick (Rhipicephalus sanguineus) and can also be transmitted by blood transfusion or contaminated needles.

Secreted antigen 1 and 3 (SAG1 and SAG3) are key antigens of Babesia gibsoni that are involved in the invasion of host red blood cells. SAG1 is a surface protein that is highly conserved among different strains of Babesia gibsoni and is a potential target for the development of vaccines and diagnostic tests. SAG3 is a secreted protein that is involved in the formation of a complex with other parasite proteins.

12D3 antigen, also known as 12-kDa, 32 kDa, 57kDa, is another important antigen of Babesia gibsoni that is involved in the invasion of red blood cells. It is a potential target for the development of vaccines and diagnostic tests.

Merozoite antigen is a group of antigens that are involved in the invasion of red blood cells by Babesia gibsoni. They are potential targets for the development of vaccines and diagnostic tests.

50 kDa surface antigen is a surface protein of Babesia gibsoni that is involved in the invasion of red blood cells. It is highly conserved among different strains of the parasite and is a potential target for the development of vaccines and diagnostic tests.

Heat shock protein 70 (HSP70) is a chaperone protein that is involved in the folding and transport of proteins in the cell. It is also a key antigen of Babesia gibsoni that is upregulated during the intraerythrocytic stage of the parasite’s life cycle. HSP70 is a potential target for the development of vaccines and diagnostic tests.

Apical membrane antigen 1 (AMA1) is a surface protein of Babesia gibsoni that is involved in the invasion of red blood cells. It is a potential target for the development of vaccines and diagnostic tests.

Interspersed repeat antigen (IRA) is another important antigen of Babesia gibsoni that is involved in the invasion of red blood cells. It is highly variable among different strains of the parasite and is a potential target for the development of vaccines and diagnostic tests.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.