Cleavable Iodoacetyl-Activated Magnetic Beads

BcMag™ Cleavable Iodoacetyl-activated Magnetic Beads are uniform, silica-based superparamagnetic beads coated with a high density of cleavable iodoacetyl functional groups on the surface. It is designed to enable fast, efficient, and covalent immobilization of protein, peptides, and other ligands through their sulfhydryl groups (-SH) for affinity purification procedures. At physiological to alkaline circumstances (pH 7.2 to 9) in either aqueous or organic solvents 20- 30% DMSO or DMF, iodoacetyl-activated supports react with sulfhydryl groups, resulting in stable thioether bonds. These reactions are often carried out in the dark to prevent the formation of free iodine, which can react with tyrosine, histidine, and tryptophan residues. Since the active iodoacetyl group is linked with the beads through a built-in cleavable disulfide linker, reducing agents such as DTT or β-mercaptoethanol can cleave and separate the target molecule-ligand complex from the beads, and only a small sulfhydryl group is attached to ligand after affinity purification. Moreover, the hydrophilic surface ensures low nonspecific adsorption, excellent dispersion, and easy handling in various buffers. The beads are suitable for conjugating larger protein or small biomolecules without steric hindrance.