Yersinia pestis cDNA and recombinant antigen

Yersinia pestis is a Gram-negative, rod-shaped, zoonotic, enterobacterium that causes the plague. It is a facultative anaerobic organism that can infect humans and other animals. It can be spread through the bite of an infected flea, direct contact with an infected animal, or by inhalation of respiratory droplets from an infected human or animal. Symptoms of infection include fever, chills, headache, and swollen lymph nodes. In more severe cases, it can cause sepsis, pneumonia, and death. Treatment includes antibiotics and supportive care. It produces several key antigens including:

One of the key proteins associated with Yersinia pestis is the Caf1M protein, which is involved in the formation of the F1 capsule antigen. This capsule is a major virulence factor that helps the bacterium to evade the host’s immune system. The F1 capsule-anchoring protein and F1 operon positive regulatory protein are also involved in the formation and regulation of the F1 capsule antigen.

Another important protein associated with Yersinia pestis is the Coagulase-fibrinolysin Plasminogen activator. This protein plays a role in the bacterium’s ability to spread throughout the host’s tissues by breaking down blood clots and extracellular matrix proteins.

The Cysteine protease yopT is another key protein associated with Yersinia pestis. This protein is involved in the suppression of the host’s immune response by cleaving several key signaling molecules within host cells.

The virulence protein yopE and the Protein kinase ypkA are also important components of Yersinia pestis. YopE is involved in disrupting the host’s cytoskeleton, while YpkA is involved in modulating the host’s immune response.

In addition to these proteins, Yersinia pestis also produces several factors that are involved in its ability to adhere to and invade host cells. The invasin protein and Surface antigen-OMP85 family are both involved in the bacterium’s ability to adhere to host cells. The pH 6 antigen Antigen 4 Adhesin and the Virulence-associated V antigen are also involved in the bacterium’s ability to invade host cells.

Yersinia pestis also produces several proteins that are involved in the delivery of virulence factors into host cells. The Yop4b, LcrE, and KIM 10 Targeted effector protein are all involved in the delivery of virulence factors, while the YopP, KIM 10 Yop targeting negative regulator, KIM 10 Yop targeting protein YopB, and KIM 10 Yop targeting protein are involved in regulating this process.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

Yersinia pestis cDNA and recombinant antigen can be used in a variety of applications. For example, they can be used to develop immunoassays or diagnostic tests for the detection of Yersinia pestis or its antigens. They can also be used for vaccine development, as well as to produce monoclonal antibodies to be used for research or therapeutic purposes. In addition, cDNA and recombinant antigens can be used in basic research to study Yersinia pestis and its pathogenesis.