Vibrio parahaemolyticus cDNA and recombinant antigen

Vibrio parahaemolyticus is a bacterium that can cause foodborne illness in humans, typically through the consumption of contaminated seafood. This bacterium produces several potential virulence factors or surface proteins that may play a role in its ability to cause disease and evade the immune system.

One of these proteins is the 16-protective antigen OMA87, also known as OmpU. This protein is located in the outer membrane of the bacterium and has been shown to be involved in V. parahaemolyticus’ ability to adhere to and invade host cells. It is also a potential target for developing vaccines and diagnostic tools for V. parahaemolyticus infections.

Another antigen produced by V. parahaemolyticus is the 16 antigen. This protein is also located in the outer membrane and is thought to play a role in the bacterium’s virulence.

The AQ3810 OmpA protein is another surface antigen produced by V. parahaemolyticus. It is involved in the bacterium’s ability to adhere to host cells and has been shown to be important for colonization in a mouse model of infection.

Finally, the AQ3810 Surface antigen is another potential virulence factor produced by V. parahaemolyticus. This protein is involved in the bacterium’s ability to resist the immune system and has been shown to be important for survival in a mouse model of infection.

Overall, understanding the virulence factors and surface proteins produced by V. parahaemolyticus is important for developing effective strategies to prevent and treat infections caused by this bacterium.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

A more reliable method of diagnosis is the use of cDNA and recombinant antigens. cDNA stands for “complementary DNA” and is a type of DNA that is complementary to a particular gene sequence. Recombinant antigens are those that have been artificially created in a laboratory. cDNA and recombinant antigen tests are used to detect the presence of Vibrio parahaemolyticus in a sample. They are more sensitive and specific than traditional methods and can be used to diagnose Vibrio parahaemolyticus enteritis with greater accuracy. In addition, they can be used to differentiate between strains.