Treponema pallidum cDNA and recombinant antigen

Treponema pallidum is a spiral-shaped, Gram-negative bacterium that is the causative agent of several diseases, including syphilis, yaws, bejel, and pinta. It is found exclusively in humans and is transmitted through close contact, such as through sexual contact, contaminated needles, and from mother to fetus during pregnancy. Treponema pallidum is highly mobile and is able to move through the mucous membranes of the body, allowing it to spread quickly. The bacterium can also survive outside of the body for several hours, increasing its infectious potential.

There are several membrane and chaperonin proteins that are considered as antigens in T. pallidum. Antigens are substances that trigger the immune system to produce antibodies against them.Some of the key antigens found in T. pallidum include:

47 kDa membrane antigen: This protein is located in the outer membrane of T. pallidum and is one of the most immunogenic antigens. It is considered to be a major antigen and is often used in diagnostic tests.

60 kDa chaperonin Cpn60: This protein is a chaperonin, which means it helps other proteins to fold correctly. It is also found in the outer membrane of T. pallidum and is a major antigen.

Antigen TpF1: This protein is specific to T. pallidum and is considered to be a good diagnostic marker for syphilis. It is often used in combination with other antigens to improve diagnostic accuracy.

Antigen C1-5: This group of five proteins is found in the cytoplasm of T. pallidum and are considered to be minor antigens. They are less immunogenic compared to the major antigens but are still used in diagnostic tests.

Antigen 4D: This protein is found in the outer membrane of T. pallidum and is considered to be a minor antigen.

Antigen p83-100: This protein is found in the cytoplasm of T. pallidum and is considered to be a minor antigen.

Membrane antigen pathogen-specific antigen: This antigen is specific to T. pallidum and is found in the outer membrane. It is often used in diagnostic tests.

Membrane lipoprotein tmpC antigen: This protein is a lipoprotein and is found in the outer membrane of T. pallidum. It is considered to be a minor antigen.

tpn50 antigen: This protein is found in the cytoplasm of T. pallidum and is considered to be a minor antigen.

Antigen tpp57: This protein is found in the cytoplasm of T. pallidum and is considered to be a minor antigen.

Treponemal membrane protein A antigen tmpA: This protein is found in the outer membrane of T. pallidum and is considered to be a minor antigen.

Treponemal membrane protein B antigen tmpB: This protein is found in the outer membrane of T. pallidum and is considered to be a minor antigen.

These antigens are important in the diagnosis of syphilis, as they can be used to detect the presence of antibodies in a patient’s blood. Additionally, some of these antigens have been studied as potential targets for vaccine development against syphilis.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

Treponema pallidum cDNA and recombinant antigens can be used for a variety of applications. These include diagnostics, vaccine development, and research.

Diagnostics – cDNA and recombinant antigens can be used in serological tests to detect Treponema pallidum antibodies in a patient’s serum. This is useful when diagnosing syphilis and other treponemal infections.

Vaccine Development – cDNA and recombinant antigens can be used to develop a vaccine against Treponema pallidum. This is an important step in the prevention of syphilis and other treponemal infections.

Research – cDNA and recombinant antigens can be used to study the structure, function, and genetics of Treponema pallidum. This can be used to develop new treatments and drugs for syphilis and other treponemal infections.