Streptococcus pneumoniae cDNA and recombinant antigen

Streptococcus pneumoniae is a pathogenic bacterium that is responsible for a wide range of diseases, including pneumonia, meningitis, and sepsis. Understanding the key components of this bacterium is essential for developing effective treatments and vaccines.

Autolysin Cell Wall Hydrolase and Choline-Binding Protein A

Autolysin cell wall hydrolase and choline-binding protein A are two important components of the Streptococcus pneumoniae cell wall. Autolysin cell wall hydrolase plays a crucial role in cell division and cell wall maintenance, while choline-binding protein A is involved in adherence to host cells and the initiation of infection.

Hyaluronate Lyase-Hyaluronidase HYase and Immunoglobulin A1 Protease

Hyaluronate lyase-hyaluronidase HYase and immunoglobulin A1 protease are two virulence factors that are produced by Streptococcus pneumoniae. HYase breaks down hyaluronic acid, which is an important component of the extracellular matrix and a key component of the host immune system. Immunoglobulin A1 protease degrades immunoglobulin A1, which is the most abundant antibody isotype found in the respiratory tract. This allows the bacterium to evade the host immune system and establish infection.

PspA and Choline-Binding Proteins

PspA and choline-binding proteins are two important components of the Streptococcus pneumoniae cell membrane. PspA is involved in immune evasion and is a potential target for vaccine development. Choline-binding proteins are involved in cell division and cell wall maintenance, as well as adherence to host cells and the initiation of infection.

Antigen Cell Wall Surface Anchor Family and Pneumolysin

Antigen cell wall surface anchor family and pneumolysin are two important components of the Streptococcus pneumoniae cell surface. Antigen cell wall surface anchor family proteins are involved in adherence to host cells and the initiation of infection. Pneumolysin is a pore-forming toxin that plays a crucial role in the pathogenesis of Streptococcus pneumoniae by damaging host cells and promoting the release of bacterial products.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

Streptococcus pneumoniae cDNA and recombinant antigens can be used in a variety of applications. The cDNA can be used to produce recombinant proteins, which can be used as vaccines or therapeutics. Recombinant antigens can also be used in diagnostic tests, such as ELISA or immunoblotting, to detect the presence of the bacteria in a sample. The cDNA can also be used for the production of monoclonal antibodies, which can be used to recognize and target the bacteria in a variety of ways. Finally, the cDNA can be used to study the genetics of the bacteria, which can provide valuable insight into its behavior and virulence.