Salmonella typhi cDNA and Antigen

Salmonella Typhi is the causative agent of typhoid fever, a serious and potentially fatal illness that is transmitted through contaminated food or water. This bacterium has several key virulence factors that enable it to invade and colonize host tissues, including the ones you mentioned.

The cell adherence-invasion protein (Cia) is a critical virulence factor that allows Salmonella Typhi to adhere to and invade host cells. This protein is involved in the initial attachment of the bacterium to the host cell surface, which is followed by the secretion of other virulence factors that enable invasion.

Flagellin is another important virulence factor that is essential for the motility of Salmonella Typhi. It enables the bacterium to move through the host’s intestinal epithelial cells, allowing it to reach other tissues and organs.

The invasion proteins InvA, InvE, and InvF are part of the type III secretion system (T3SS) that is critical for the invasion and survival of Salmonella Typhi within host cells. These proteins are responsible for the secretion of bacterial effector proteins that manipulate the host cell machinery to the advantage of the bacterium.

Finally, Salmonella Typhi produces various secretory proteins that aid in its invasion and survival within the host. These proteins include SipA, SipB, SipC, SipD, SipE, and SopB, among others.

Understanding these virulence factors is crucial for developing effective diagnostic tests, therapies, and vaccines to combat Salmonella Typhi infections.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

The application of Salmonella typhi cDNA and recombinant antigen for the diagnosis of typhoid fever is an effective tool for detecting the presence of the bacteria in the body. This method can detect the presence of the bacteria in the blood, stool, or other body fluids. The cDNA and recombinant antigens can detect both S. typhi and its variants. This allows for a more accurate diagnosis of typhoid fever. The cDNA and recombinant antigens can be used to identify the exact strain of S. typhi and thus provide more specific treatment for the infection. Additionally, the use of cDNA and recombinant antigens can reduce the time required for diagnosis and treatment of typhoid fever.