Burkholderia pseudomallei cDNA and Antigen

Burkholderia pseudomallei is a bacteria species that is known to cause melioidosis, a severe and potentially fatal disease. It is a Gram-negative, rod-shaped, facultative anaerobe that is found in soil and water. It is endemic in certain parts of Southeast Asia and northern Australia, but it has been found in other parts of the world as well. Treatment for melioidosis is complicated and often involves a long course of antibiotics.
The bacterium is known to have several surface antigens that are important for its virulence and pathogenicity. In this article, we will discuss some of the most significant Burkholderia pseudomallei surface antigens.

p200 Antigen:

The 200 kDa antigen, also known as p200, is one of the most important surface antigens of Burkholderia pseudomallei. This antigen is a heat-modifiable protein and is expressed by most strains of the bacterium. p200 is thought to be important for the bacterium’s adhesion and invasion of host cells.

30-kDa Protein Antigen:

The 30-kDa protein antigen is another significant surface antigen of Burkholderia pseudomallei. This antigen is found in both the cytoplasm and the outer membrane of the bacterium. The 30-kDa protein is also known to be highly immunogenic and has been used as a diagnostic marker for melioidosis.

Rickettsia 17 kDa Surface Antigen:

The Rickettsia 17 kDa surface antigen is a highly conserved protein found in several bacterial species, including Burkholderia pseudomallei. This antigen is thought to be involved in the attachment and entry of the bacterium into host cells.

Sm65 Antigen:

The Sm65 antigen is a surface protein found in many Burkholderia pseudomallei strains. This antigen is highly immunogenic and has been shown to be protective against the bacterium in animal models. Sm65 has also been used as a diagnostic marker for melioidosis.

Surface Antigen Protein:

The surface antigen protein is a large family of proteins found on the surface of many bacterial species, including Burkholderia pseudomallei. These proteins are known to be involved in a variety of functions, including adhesion, invasion, and immune evasion.

In conclusion, understanding the surface antigens of Burkholderia pseudomallei is critical for the diagnosis and treatment of melioidosis. The p200 antigen, 30-kDa protein antigen, Rickettsia 17 kDa surface antigen, Sm65 antigen, and surface antigen proteins are just a few of the many important antigens expressed by this bacterium. By studying these antigens, researchers can gain insight into the pathogenesis of melioidosis and develop new strategies for the prevention and treatment of this deadly disease.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.