Brucella ovis cDNA and Antigen

Brucella ovis is a species of bacteria in the genus Brucella. It is the causative agent of ovine brucellosis, a disease of sheep which can cause infertility, abortion, and reduced milk production. It is primarily transmitted through contact with infected animals or contaminated products such as animal products, semen, and needles. Treatment of the disease is difficult, and prevention is the most effective approach. Vaccination is available for use in some countries.

Brucella ovis produces a range of membrane antigens and proteins that play important roles in its survival and pathogenesis. Here’s a closer look at some of these key membrane antigens and proteins:

Omp25c: This is a major outer membrane protein of Brucella ovis that is highly conserved across different species of Brucella. Omp25c is known to be a potent immunogen and is often used in serodiagnostic tests for brucellosis.

OmpA: Another outer membrane protein of Brucella ovis, OmpA has been shown to be essential for the bacterium’s ability to invade and survive within host cells. It is also a strong immunogen and has been proposed as a potential vaccine candidate.

Omp31: This is a porin protein that forms part of the outer membrane of Brucella ovis. It has been shown to be involved in the regulation of bacterial cell division, as well as in the bacterium’s ability to resist the host immune response.

Tyrosine recombinase XerD: This is a membrane-associated protein that plays a key role in the maintenance and segregation of the Brucella ovis chromosome during cell division.

Urease: Urease is an enzyme produced by Brucella ovis that plays a key role in the bacterium’s survival within the acidic environment of the host’s phagolysosome. It accomplishes this by neutralizing the acidic pH through hydrolysis of urea.

In summary, Brucella ovis produces a range of membrane antigens and proteins that are essential for its survival and pathogenesis. These antigens and proteins may have potential applications in the development of serodiagnostic tests and vaccines for brucellosis.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

The cDNA (complementary DNA) and recombinant antigens of Brucella ovis have various applications in the fields of molecular biology and veterinary medicine, including:

Gene expression analysis: The cDNA can be used as a template to synthesize complementary RNA (cRNA) which can then be used for microarray or RNA sequencing (RNA-seq) analysis to study gene expression patterns in the bacterium under different conditions.

Antimicrobial resistance studies: The cDNA can be used to study the mechanisms of antibiotic resistance in Brucella ovis, including the identification of genes involved in resistance and the evaluation of the expression of these genes.

Vaccine development: The recombinant antigens of Brucella ovis can be used to develop subunit vaccines against the bacterium. These antigens can stimulate the immune system to mount a response against the pathogen.

Diagnostics: The cDNA can be used to develop real-time PCR (polymerase chain reaction) assays for the rapid and sensitive detection of Brucella ovis in clinical specimens, such as blood, synovial fluid, and semen.

Serological assays: The recombinant antigens of Brucella ovis can be used to develop serological assays, such as ELISA (enzyme-linked immunosorbent assay), to detect the presence of antibodies against the bacterium in sheep serum. These assays can be used for the diagnosis of Brucella ovis infections in livestock.