Brucella abortus cDNA and Antigen

Brucella Abortus is a gram-negative bacterium that causes Brucellosis in cattle and other animals. It is also known to be transmitted to humans through contact with infected animals or their products. The bacteria have several key proteins that play a vital role in the immune response to Brucellosis. In this article, we will explore some of the most important proteins of Brucella Abortus and their functions.

Chaperonin Cpn60

Chaperonin Cpn60 is a 60 kDa protein that acts as a molecular chaperone. It assists in the proper folding and assembly of other proteins in the cell. This protein is critical to the survival of the bacteria and is also important in the immune response to Brucellosis.

BacA Bacteroid Development Prot

BacA Bacteroid Development Prot is a protein that plays a crucial role in the development of Brucella Abortus in the host cell. It helps the bacteria to evade the immune system and survive within the host. It is considered a key virulence factor for the bacteria.

CD9-CD37-CD63 Antigen

CD9-CD37-CD63 Antigen is a surface protein of Brucella Abortus that plays a role in the adherence of the bacteria to host cells. It is also known to induce the production of inflammatory cytokines, which helps in the immune response to the bacteria.

Omp25d and Lipoprotein Omp19

Omp25d and Lipoprotein Omp19 are two immunoreactive proteins of Brucella Abortus. Omp25d is known to be a virulence factor and is essential for the survival of the bacteria within the host. Lipoprotein Omp19, on the other hand, plays a role in the immune response to the bacteria and is a potential target for the development of vaccines.

RfbE O-Antigen Export System ATP-Binding Protein RfbE

RfbE O-Antigen Export System ATP-Binding Protein RfbE is a protein that is involved in the export of O-Antigen, which is a component of the bacterial cell wall. It is essential for the survival of the bacteria within the host and is also a potential target for the development of vaccines.

Surface Antigen

Surface Antigen is a protein that is present on the surface of Brucella Abortus. It plays a critical role in the interaction of the bacteria with the host cell and is also a potential target for the development of vaccines.

Tyrosine Recombinase Xer

Tyrosine Recombinase Xer is a protein that is involved in the recombination of DNA. It is essential for the survival of the bacteria and is also important in the immune response to Brucellosis.

Uncharacterized Protein BruAb2_0312

Uncharacterized Protein BruAb2_0312 is a protein of Brucella Abortus that has not been fully characterized. Its function is not yet known, but it is a potential target for further research.

Urease Subunit Beta 1 and Gamma 1

Urease Subunit Beta 1 and Gamma 1 are two subunits of the urease enzyme of Brucella Abortus. Urease plays a crucial role in the survival of the bacteria within the host.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.

The cDNA (complementary DNA) and recombinant antigens of Brucella abortus have various applications in the fields of molecular biology and infectious disease, including:

Gene expression analysis: The cDNA can be used as a template to synthesize complementary RNA (cRNA) which can then be used for microarray or RNA sequencing (RNA-seq) analysis to study gene expression patterns in the bacterium under different conditions.

Antimicrobial resistance studies: The cDNA can be used to study the mechanisms of antibiotic resistance in Brucella abortus, including the identification of genes involved in resistance and the evaluation of the expression of these genes.

Vaccine development: The recombinant antigens of Brucella abortus can be used to develop subunit vaccines against the bacterium. These antigens can stimulate the immune system to mount a response against the pathogen.

Diagnostics: The cDNA can be used to develop real-time PCR (polymerase chain reaction) assays for the rapid and sensitive detection of Brucella abortus in clinical specimens, such as blood, synovial fluid, and cerebrospinal fluid.

Serological assays: The recombinant antigens of Brucella abortus can be used to develop serological assays, such as ELISA (enzyme-linked immunosorbent assay), to detect the presence of antibodies against the bacterium in human or animal serum. These assays can be used for the diagnosis of Brucella abortus infections.