Woolly monkey hepatitis B virus cDNA and Antigen

Woolly monkey hepatitis B virus (WMHBV) is a virus that infects woolly monkeys and is closely related to human hepatitis B virus. It is a member of the Hepadnavirus genus, which includes viruses that cause liver inflammation in humans, non-human primates, and other animals. WMHBV is the only known virus in this genus to infect woolly monkeys. The virus is endemic in some wild populations of woolly monkeys and has also been found in captive individuals. WMHBV infection causes a mild, self-limiting form of hepatitis in woolly monkeys, but can also lead to chronic infection and liver disease. Control of WMHBV in captive populations is important to prevent the spread of the virus to humans and other non-human primates.

Woolly monkey hepatitis B virus (WMHBV) antigen is a type of antigen found in some members of the genus Lagothrix, including the woolly monkeys. It is a member of the family Hepadnaviridae, which also includes the human hepatitis B virus. WMHBV antigen is found in both serum and liver tissue of infected animals and is used in diagnostic tests to detect infection with WMHBV.

Woolly monkey hepatitis B virus (WMHBV) is a member of the Orthohepadnavirus genus from the family of Hepadnaviridae. It is a single-stranded DNA virus that infects woolly monkeys (Lagothrixpoeppigii), a species of New World monkeys found in the Amazon rainforest. The WMHBV genome is approximately 3.2 kb in length and contains the four core genes found in other hepadnaviruses: the preS/S gene, the core gene, the polymerase gene, and the X gene. The preS/S gene encodes the envelope proteins, the core gene encodes the core antigen, the polymerase gene encodes the DNA-dependent DNA polymerase, and the X gene encodes the X (or e) antigen. Additionally, the WMHBV genome has been found to contain four unique accessory genes: the V gene, the W gene, the Y gene, and the Z gene. The V gene encodes an unknown protein, the W gene encodes a putative transcriptional regulator, the Y gene encodes a putative reverse transcriptase, and the Z gene encodes an unknown protein.

The capsid protein of WMHBV, also known as core protein, forms the viral nucleocapsid and plays an important role in viral replication. The external core antigen, or e-antigen, is a secreted form of the core protein that is thought to suppress the host immune response to the virus. The large envelope protein is responsible for binding to host receptors and mediating virus entry into host cells.

Studies of WMHBV have provided valuable insights into the biology of hepatitis B viruses and their interactions with host cells. The virus is also used as a model system for developing new antiviral therapies for human hepatitis B infections.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.