Moloney murine leukemia virus cDNA and Antigen

Moloney murine leukemia virus is a retrovirus that infects mice and has been widely used as a model system for studying retroviral replication and gene expression. Its key proteins include the Glycosylated Gag polyprotein, Matrix protein p15, Capsid protein p30, Integrase p46, and Protease p14.

Moloney murine leukemia virus (MoMLV) is a retrovirus that is commonly used in gene therapy and molecular biology research. The virus is named after its discoverer, John Moloney, and causes leukemia and lymphoma in mice.

The MoMLV genome encodes several structural and non-structural proteins that are involved in the virus life cycle. One of the most important proteins is the glycosylated Gag polyprotein, which is processed to generate the matrix protein p15 and capsid protein p30. These proteins are critical for the assembly of the virus particle.

Another important protein is the integrase p46, which mediates the integration of the viral DNA into the host genome. The protease p14 is responsible for cleaving the Gag and Gag-Pol polyproteins into their individual components, allowing for the assembly of new virus particles.

Understanding the function of these proteins is important for developing effective antiviral therapies and for improving the use of MoMLV as a tool for gene therapy and molecular biology research.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.