Infectious hypodermal and hematopoietic necrosis virus cDNA and Antigen

Infectious hypodermal and hematopoietic necrosis virus (IHHNV) is a virus that affects the health of penaeid shrimp, including species such as Pacific white shrimp and black tiger shrimp. IHHNV causes a disease known as infectious hypodermal and hematopoietic necrosis (IHHN), which is characterized by symptoms such as stunted growth, reduced survival, and decreased production in infected shrimp populations. IHHNV is an important pathogen in the shrimp aquaculture industry and has caused significant economic losses globally. Control and prevention measures, such as proper biosecurity and vaccination, are essential in reducing the spread and impact of IHHNV.

The infectious hypodermal and hematopoietic necrosis virus (IHHNV) antigen refers to a protein or substance found in or produced by the virus that triggers an immune response. The IHHNV antigen can be used in the development of diagnostic tests to detect the presence of the virus in shrimp populations. Additionally, the production of recombinant IHHNV antigen can be used as a component in the creation of vaccines to prevent IHHNV infection and the associated disease, infectious hypodermal and hematopoietic necrosis (IHHN). The detection of IHHNV antigen is an important tool in monitoring and controlling the spread of the virus in shrimp aquaculture.

The infectious hypodermal and hematopoietic necrosis virus (IHHNV) genome refers to the complete set of genetic material present in the virus. The genome of IHHNV is composed of DNA (deoxyribonucleic acid) and is characterized by its unique nucleotide sequence. The study of the IHHNV genome is important in understanding the biology of the virus, its pathogenesis, and its evolution over time. The information derived from the IHHNV genome can be used to develop better diagnostic tools, vaccines, and control measures for IHHNV infection in shrimp populations. Additionally, the analysis of the IHHNV genome provides insight into the molecular mechanisms involved in the replication and spread of the virus, which is important for the development of antiviral strategies.

Infectious hypodermal and hematopoietic necrosis virus (IHHNV) is a single-stranded DNA virus that can cause significant mortality in shrimp, leading to significant economic impacts on the shrimp aquaculture industry. The virus contains several key proteins that are essential for its replication and pathogenesis. Three of these proteins are:

NS2 protein: The NS2 protein of IHHNV is a non-structural protein that plays a role in virus replication. It is also involved in the inhibition of the host immune response.

Viral capsid protein: The viral capsid protein forms the outer shell of the virus particle and is essential for the protection of the viral genome. It is also involved in the assembly of the virus particle.

37 kDa coat protein: The 37 kDa coat protein is another structural protein of IHHNV that is involved in virus assembly and maturation. It is also thought to play a role in the host immune response.

Understanding the functions and interactions of these proteins is important for developing strategies for the prevention and control of IHHNV infections in shrimp populations. Research on these proteins may also have broader implications for the aquaculture industry as a whole, given the significant impact of IHHNV and other viral pathogens on the economic viability of shrimp production.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E. coli expression Vector), which are ready for production of the recombinant proteins.