Human coronavirus cDNA and Antigen

Human coronavirus is a type of virus that can cause respiratory infections, ranging from the common cold to severe diseases such as Middle East Respiratory Syndrome (MERS) and severe acute respiratory syndrome (SARS). Some strains of human coronavirus have recently been identified as the cause of a global pandemic.

The genome of a virus is its genetic material, which determines its characteristics and is encoded in either RNA or DNA. The genome of human coronaviruses (such as SARS-CoV-2, which causes COVID-19) is a single-stranded RNA molecule of positive polarity. It encodes several important proteins, including the spike protein that allows the virus to infect host cells and the replicase enzymes that enable the virus to replicate its genetic material. The study of the human coronavirus genome has played a crucial role in understanding the biology and evolution of these viruses and in the development of diagnostic tools, vaccines, and antiviral treatments.The viruses are characterized by their distinctive spike proteins, which are responsible for their distinctive crown-like appearance.

The 229E nucleoprotein is a structural protein that plays a role in the packaging and replication of the viral genome. The 229E spike glycoprotein is responsible for viral attachment and entry into host cells, making it an important target for antiviral therapies and vaccine development.

The HKU1 hemagglutinin-esterase glycoprotein is a multifunctional protein that is thought to play a role in host cell recognition, viral entry, and viral replication. Research is ongoing to better understand the precise roles of this protein in the coronavirus life cycle.

The HKU1 spike protein S2 is another key component of the coronavirus spike protein complex, which is responsible for viral attachment and entry into host cells. Recent research has shown that this protein plays a critical role in the fusion of the viral and host cell membranes during viral entry.

Overall, understanding the molecular and structural features of these key viral proteins is critical for developing effective diagnostic tools, antiviral therapies, and vaccines for human coronavirus infection. Continued research into the function and expression of these proteins will help to improve our understanding of the virus and develop new strategies for controlling its spread.

The use of recombinant proteins/cDNA in academic research and therapeutic applications has skyrocketed. However, in heterologous expression systems, successful recombinant protein expression is dependent on a variety of factors, including codon preference, RNA secondary structure, and GC content. When compared to pre-optimization, more and more experimental results demonstrated that the expression level was dramatically increased, ranging from two to hundred times depending on the gene. Bioclone has created a proprietary technology platform that has resulted in the creation of over 6,000 artificially synthesized codon-optimized cDNA clones (cloned in E.coli expression Vector), which are ready for production of the recombinant proteins.