- +1 858 909 0079
- +1 858 909 0057
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- +1 858 909 0079
- [email protected]
Cat. No.
Product Name
Unit Size
Order
Specification
Composition
Magnetic beads are grafted with a Sulfhydryl group on the surface
Number of Beads
~ 1.68 x 109 beads/mg (1μm beads)
~ 5 x 107 beads /mg (5μm beads)
Magnetization
~40-45 EMU/g
Type of Magnetization
Superparamagnetic
Effective Density
2.5 g/ml
Formulation
Lyophilized Powder
Functional Group Density
1μm Magnetic Beads
~250 μmole / g of Beads
5μm Magnetic Beads
~200 μmole / g of Beads
1μm Long-Arm Magnetic Beads
~220 μmole / g of Beads
5μm Long-Arm Magnetic Beads
~185 μmole / g of Beads
Storage
Upon receipt, store at 4°C
A sulfhydryl is a functional group that contains a sulfur atom linked to a hydrogen atom. In chemistry, the sulfhydryl group, often known as a thiol, is denoted by the suffix “-thiol” and the prefix “mercapto-” or “sulfanyl.” Thiols have a strong preference for soft metals.
BcMag™ Sulfhydryl-Terminated Magnetic Beads are uniform, silica-based superparamagnetic beads grafted with a high density of sulfhydryl (SH) functional groups on the surface. The Sulfhydryl-Magnetic Beads are specially designed for conjugation of protein/peptides modified by SMCC (Sulfosuccinimidyl 4-[N-maleimidomethyl] cyclohexane-1-carboxylate). SMCC contains an amine-reactive N-hydroxysuccinimide (NHS ester) and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimides react with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. The procedure is quick and straightforward for sample preparation without laborious repeat pipetting and centrifuging. BcMagTM Sulfhydryl-Terminated Magnetic Beads are ideal for conjugating large proteins. BcMagTM Long-Arm Sulfhydryl-Terminated Magnetic Beads are recommended for conjugating small peptides.
BcMag™ Sulfhydryl-Terminated Magnetic Beads work perfectly as solid resin for various affinity purifications to refine molecules, cells, and parts of cells into purified fractions. After conjugation with ligands, add the beads to a solution containing the target molecules, then mix, incubate, wash and elute the target molecules.
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