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Products
One-Step NGS Cleanup Kit
Introduction
Next-generation sequencing (NGS), also called massively parallel sequencing, is a high-throughput, quick, and scalable method that can replace first-generation Sanger sequencing. It enables the discovery and analysis of various types of genomic features in a single sequencing run, ranging from single nucleotide variants (SNVs) to copy number and structural variants, making it a quick and cost-effective tool for genomic research.
BcMag™ One-Step NGS Cleanup Kit is specially designed for ultrafast and efficient purification of DNA after adaptor ligation and PCR or possible replacement of size selection procedure after adaptor addition. The protocol is not only straightforward (one tube and one step, as shown in Fig.1) but also very flexible in removing different size DNA fragments by adjusting processing time, buffer’s pH, and detergent concentration (table1). The magnetic Beads are added directly to the finished PCR reactions or other DNA reactions and mixed by a vortex mixer or pipetting to capture and remove the impurities (e.g., excess primer, dimer, adapter, salt, detergent, dNTPs, and enzyme). After mixing, the beads are magnetically removed, while the supernatant contains the purified and ready-to-run DNA. In just 1 minute, the purified DNA is ready for downstream applications, such as Sanger Sequencing, Restriction Digestion, Cloning, SNP Detection, or Library Preparation for NGS. The beads enable 96 samples to be processed simultaneously in less than 10 minutes.
Features and Advantages
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Simple protocol: No liquid transfer, One-tube, One-step
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Ultrafast: One-minute protocol
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Higher purity and recovery > 90% DNA
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Effective Cleanup: Removes excess primer (<100- mer ssDNA), dimer, adapter, a salt such as Mg2+, detergent, dNTPs, enzymes, and dye.
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Cost-effective: Eliminates columns, filters, laborious repeat pipetting, and ethanol
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High throughput: Compatible with many different automated liquid handling systems