- +1 858 909 0079
- +1 858 909 0057
- [email protected]
- +1 858 909 0079
- [email protected]
Cat. No.
Product Name
Unit Size
Order
Specification
Composition
Magnetic beads coated with a high density of cleavable amine group on the surface
Number of Beads
~ 1.68 x 109 beads/mg (1μm beads)
~1.47 x 108 beads/mg (2.5μm beads)
Stability
Short Term (<1 hour): pH 3-11; Long-Term: pH 4-10
Temperature: 4°C -140°C; Most organic solvents
Magnetization
~40-45 EMU/g
Type of Magnetization
Superparamagnetic
Concentration
30 mg/ml (1 mM ETDA, pH 7.5)
Functional Group Density
1μm Magnetic Beads
~250 μmole / g of Beads
2.5μm Magnetic Beads
~240 μmole / g of Beads
Storage
Store at 4°C upon receipt. Do not freeze.
BcMag™ Cleavable Amine-Terminated Magnetic Beads are magnetic beads coated with a high density of primary amine functional groups on the surface. The beads can covalently conjugate primary amine or carboxy-containing ligands such as protein and peptides for affinity purification. Since the active amine group is linked with the beads through a built-in cleavable disulfide linker (Fig.1), reducing agents such as DTT or β-mercaptoethanol can cleave and separate the target molecule-ligand complex from the beads. Moreover, the hydrophilic surface ensures beads low nonspecific adsorption, excellent dispersion, and easy handling in various buffers. The beads are suitable for the conjugation of large-size proteins or small peptides.
The amine beads work perfectly as affinity resin for affinity purification to refine molecules, cells, and parts of cells into purified fractions. After conjugation with ligands, add the beads to a sample containing the target molecules, then mix, incubate, wash and elute the target molecules.
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Covalently coupled with high efficiency
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Easy to use
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Stable covalent bond with low levels of ligand leakage
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Produces reusable immunoaffinity matrix
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Low nonspecific binding
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Immobilize 1-10 mg protein or 0.1-1 mg peptide/ml beads
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A cleavable built-in disulfide bond allows the ligand-target molecule complex to separate from the beads.
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Application: Purification for antibody, protein/peptide, DNA/RNA; cell sorting, immunoprecipitation
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