- +1 858 909 0079
- +1 858 909 0057
- [email protected]
- +1 858 909 0079
- [email protected]
Products
One-Step Mammalian Cell DNA Purification Kit
Components
Previous slide
Next slide
Shipping conditions: At ambient temperature
Handling And Storage: Store The Kit Components According To The Table Above On Arrival.
Components
BcMag™ U-DNA Beads
10x Lysis Buffer (100mM Tris-HCl, PH 9.0)
Proteinase K
DTT
Proteinase K Suspension Buffer
Storage
4°C
4°C
-20°C
-20°C
4°C
Cat. No. AB101 (50 Preps)
2.5 ml
0.6 ml
12.5 mg
15.4 mg
1.0 ml
Cat. No. AB102 (100 Preps)
5.0 ml
1.2 ml
25 mg
30.8 mg
2.0 ml
Shipping conditions: At ambient temperature
Handling and Storage: Store the kit components according to the table Above on arrival.
Introduction
Large-scale purification of high-quality DNA from mammalian cells in sufficient quantities requires an efficient methodology for high sample volumes. Nevertheless, the protocols for most commercially available genomic DNA (gDNA) extraction kits suffer from a tedious binding-washing-elution step, poor integrity, DNA losses, and the use of toxic organic solvents. To overcome these drawbacks, we developed a novel revolutionary one-step DNA purification system based on magnetic beads and negative chromatography, which combines DNA extraction with removing all the PCR inhibitors from the samples without performing DNA isolation and purification steps.
BcMag™ One-Step Mammalian Cell DNA Purification Kit allows rapid and efficient purification of genomic DNA from cells. It uses novel negative selection chromatography magnetic beads to quickly capture impurities such as PCR inhibitors from cell lysate (See “PCR inhibitor removal”), leaving the DNA untouched. It reduces the risk of DNA loss and carryover of extraction buffers from the traditional and tedious bind-wash-elute procedure. The purification kit provides a fast and simple method for DNA extraction with only one tube, no liquid transfer, and no requirement for carrier RNA. After preparing the lysates, it enables the processing of 96 samples in less than 15 minutes, with less than 1 minute of hands-on Time.
Principle and Workflow
1.
Add functional magnetic beads to the sample.
2.
Mix the samples with the magnetic beads and proteinase K to lyse the cells.
3.
Mix by vertexing/pipetting for the beads to capture the PCR inhibitors.
4.
Remove the beads with a magnet.
5.
Aspirate the supernatant containing the pure ready-to-use DNA.
Performance
The purified DNA is suitable for use in sensitive downstream applications, such as PCR, qPCR, single-nucleotide polymorphism (SNP), short tandem repeat (STR) genotyping, genotyping, or next-generation sequencing (NGS), etc.
Features and Advantages
●
Rapid and efficient purification protocol: without prior DNA isolation for subsequent use in direct workflows, No liquid transfer, and One-tube.
●
Ultrafast: Process 96 samples in less than an hour.
●
Highest nucleic acids recovery rates: Minimal loss of DNA during extraction.
●
Effectively removes inhibitors: polyphenolic compounds, humic/fulvic acids, acidic polysaccharides, tannins, melanin, heparin, detergents, denim dyes, divalent cations such as Ca2+, Mg2+, etc.
●
Cost-effective: Eliminates columns, filters, laborious repeat pipetting, and organic reagents.
●
High throughput: Compatible with many different automated liquid handling systems.